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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Cell Biol. 2011 Jul 24;13(8):989–995. doi: 10.1038/ncb2292

Figure 5. Expression of MAL G-actin binding motifs (RPEL) rescues the Lpd knockdown orientation and positioning defects of bipolar pyramidal neurons.

Figure 5

Mouse embryos were electroporated in utero at E14.5 and harvested at E18.5. (a) Schematic representation of SRF/MAL activity upon RPEL-NLS23 rescue of the Lpd knockdown. (b) Percentage of bipolar tangentially oriented cells in the IZ/SVZ of samples co-electroporated with Lpd knockdown vector and RPEL-NLS or RPEL(*)-NLS containing RPEL motif (mutations) ** p <0.01, Student’s t-test, n = 3 brains per condition). Bar graphs are plotted as mean ± SEM. (c) Quantification of cell distribution in cortical sections co-electroporated with Venus, Lpd shRNA and either RPEL-NLS or RPEL(*)-NLS containing mutations in the RPEL motif that disrupts G-actin binding. (* p <0.05, **p <0.01, *** p <0.001, Student’s t-test, n = 3 brains per condition). Scale bars: 50 μm. Bar graphs are plotted as mean ± SEM.