. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: Mol Cell Biochem. 2011 May 4;355(1-2):135–148. doi: 10.1007/s11010-011-0847-9

Table 3. Binding affinity and intermolecular distance between Cy3-labeled CPTI peptides and Cy5-labeled LCFA-CoA binding proteins: FRET results.

Values were calculated based upon the sensitized emission of acceptor (Cy5) fluorescence as a function of increasing acceptor concentration. Values represent the mean value ± the standard error, n = 4. N.D. stands for not detected.

FRET pair		Sensitized Emission
Donor	Acceptor	K_d (nM)	R (Å)
CPTI WT	ACBP	5 ± 1	56 ± 1
	L-FABP	2.5 ± 0.8	56 ± 3
	SCP-2	8 ± 2	61.5 ± 0.9
	CytoC	N.D.	N.D.
W391A	ACBP	6 ± 1	65 ± 1
	L-FABP	3.0 ± 0.6	50 ± 2
	SCP-2	7 ± 1	49 ± 2
	CytoC	N.D.	N.D.
W452A	ACBP	53 ± 9	65.8 ± 0.2
	L-FABP	28 ± 5	82 ± 1
	SCP-2	30 ± 10	68 ± 2
	CytoC	N.D.	N.D.