Table 4. CPTI activity with BSA, ACBP, L-FABP, or SCP-2 as the LCFA-CoA donor.

Mitochondria were isolated from the yeast strains separately expressing the wild-type and mutant CPTIs and then assayed for CPTI activity (as described in the Materials and Methods section) in the presence of 15, 30, 60µM, respectively of each of the LCFA-CoA donor proteins at a molar ratio of palmitoyl-CoA to protein of 7.41, 3.70, and 1.85. Values represent the means ± standard deviation, n=5.

Protein	Concentration	WT	W391A	W452A
		nmol•min-1•mg-1
BSA	15uM	33.7 ± 2.8	6.6 ± 0.2	1.3 ± 0.3
	30uM	24.3 ± 0.4	7.0 ± 0.1	1.6 ± 0.2
	60uM	15.9 ± 0.3	4.4 ± 0.3	1.2 ± 0.2
ACBP	15uM	31.8 ± 2.1	3.3 ± 0.1	0.7 ± 0.2
	30uM	27.6 ± 2.4	4.5 ± 0.9	0.8 ± 0.2
	60uM	13.2 ± 0.1	5.7 ± 0.1	1.3 ± 0.1
L-FABP	15uM	39.3 ± 2.5	4.2 ± 0.1	0.9 ± 0.2
	30uM	37.6 ± 0.1	5.9 ± 0.1	1.1 ± 0.1
	60uM	34.0 ± 0.1	7.4 ± 0.1	1.6 ± 0.1
SCP-2	15uM	31.7 ± 1.1	4.7 ± 0.1	0.8 ± 0.2
	30uM	26.1 ± 1.1	5.7 ± 0.2	1.2 ± 0.2
	60uM	16.5 ± 0.1	4.5 ± 0.1	0.9 ± 0.2