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Values were calculated based upon the decrease in donor fluorescence (CPTI) as a function of increasing acceptor concentration. Values represent the mean value ± the standard error, n = 5.
| Donor | Donor Emission | |
|---|---|---|
| Kd (nM) | R (Å) | |
| CPTI WT | 35 ± 10 | 28 ± 1 |
| W391A | 88 ± 9 | 23.9 ± 0.6 |
| W452A | 51 ± 5 | 24.3 ± 0.6 |
