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Purification of T, B cells from 4T1 TDLN and the phenotype of these cells post in vitro activation and expansion. Unfractionated TDLN cells prepared from freshly harvested 4T1 TDLNs and the purified TDLN B cells and T cells were double-stained with FITC-anti-CD3 and PE-anti-CD19 (Day 0). Activated and expanded cells were double stained similarly (Day 4). Data are representative of three experiments performed.
