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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Arthritis Rheum. 2011 Aug;63(8):2318–2328. doi: 10.1002/art.30421

Figure 1. Generation of AAV-VEGF-C and long-term expression of an AAV-encoded gene after intra-articular injection.

Figure 1

293T cells were infected with various amounts of AAV-hVEGF-C or control AAV-Luc for 48 hours. (A) The expression of hVEGF-C mRNA and (B) protein in conditioned media was assessed by real time RT-PCR or Western blot analysis. 2.5-month-old TNF-Tg mice (N=3) received an intra-articular injection of 1010 particles of AAV-Luc in the left knee joint and an intramuscular injection of 5×109 particles of the same virus in the quadriceps muscle of the right leg on day 0. Bioluminescent images were taken at various times up to 120 days. (C) Bioluminescent images of a representative mouse show persistent high Luc signals at the injected sites. (D) The mean numbers of detected light units (+SD) in each image from 3 mice. (E) At the end of the experiment (day 120), various tissues were harvested and luciferase activity was measured. Values are the means ± SD of 3 mice.