Table 2.

Primers and Enzymes for PCR-RFLP

Gene	Forward-Primer (5′L-3′L)	Reverse-Primer (5′L-3′L)	Enzyme	Annealing
FCGR2A H131R	GGAAAATCCCAGAAATTCTCGC	CAACAGCCTGACTACCTATTACGCGGG	BstU I	55°
FCGR3A V158F	CTGAAGACACATTTTTACTCCCAAA/C	TCCAAAAGCCACACTCAAAGAC	n.a.	64°
VEGF C+936T	AAGGAAGAGGAGACTCTGCGCAGAGC	TAAATGTATGTATGTGGGTGGGTGTGTCTACAGG	Nla III	60°
EGF A+61G	CATTTGCAAACAGAGGCTCA	TGTGACAGAGCAAGGCAAAG	Alu I	60°
EGFR (CA)n Repeat	ACCCCAGGGCTCTATGGGAA	TGAGGGCACAAGAAGCCCCT	n.a.	55°
EGFR G+497A	TGCTGTGACCCACTCTGTCT	CCAGAAGGTTGCACTTGTCC	Bst-N I	59°
MTHFR C677T	GCACTTGAAGGAGAAGGTGTCT	TGTGTCAGCCTCGAAAGAAAAGCT	Hin fI	68°
IL8 T-251A	TTGTTCTAACACCTGCCACTCT	GGCAAACCTGAGTCTCACA	Mfe I	60°
MTHFR A-1298C	GGAGGAGCTGCTGAAGATGTG	CCCGAGAGGTAAAGAACAAAGACTT	Mbo II	68°
COX-2 G-765C	ATTCTGGCCATCGCCGCTTC	CTCCTTGTTTCTTGGAAA GAGACG	Aci I	55°
Cyclin D1A+870G	GTGAAGTTCATTTCCAATCCGC	GGGACATCACCCTCACTTAC	ScrF I	61°
COX-2 G-765C	ATTCTGGCCATCGCCGCTTC	CTCCTTGTTTCTTGGAAA GAGACG	Aci I	55°
XRCC3Thr241Met	GCCTGGTGGTCATCGACTC	ACAGGGCTCTGGAAGGCACTGCTCAGCTCACGCACC	Nco I	60°
TS 3′UTR 6bp+/−	CAAATCTGAGGGAGCTGAGT	CAGATAAGTGGCAGTACAGA	Dra I	58°
TS 5′UTR 2R/3R	GAAAAGGCGCGCGGAAGG	GCTCCGAGCCGGCCACA	n.a	62°
RAD G51C	TGGGAACTGCAACTCATCTGG	GCGCTCCTCTCTCCAGCAG	Mva I	60°
KRAS let-7 lcs6	TGACTGAATATAAACTTGTGGTAGTTG	TCGTCCACAAAATGATTCTGAA	Tfi I	60°