Table 1.
c-Fos expression after (15 CS presentations) extinction retrieval in mice fed control or ZnR diets 3 weeks prior to conditioning
| Region (distance from bregma) | Unconditioned |
Conditioned |
Strain–diet–protocol interaction | ||||||
|---|---|---|---|---|---|---|---|---|---|
| S1 |
B6 |
S1 |
B6 |
||||||
| Ctl | ZnR | Ctl | ZnR | Ctl | ZnR | Ctl | ZnR | ||
| Cortical regions | |||||||||
| M1, 1.78 mm | 0.5 ± 0.2 | 0.6 ± 0.2 | 0.4 ± 0.1 | 0.4 ± 0.2 | 0.6 ± 0.3 | 0.6 ± 0.3 | 0.5 ± 0.3 | 0.4 ± 0.2 | F(1, 40) = 0.01, p = 0.98 |
| M2, 1.78 mm | 0.8 ± 0.4 | 0.8 ± 0.3 | 0.3 ± 0.1 | 0.5 ± 0.1 | 0.8 ± 0.2 | 0.7 ± 0.2 | 0.7 ± 0.5 | 0.5 ± 0.2 | F(1, 40) < 0.01, p = 0.97 |
| Cg1, 1.78 mm§ | 1.4 ± 0.2 | 1.4 ± 0.3 | 0.8 ± 0.3 | 0.8 ± 0.4 | 9.8 ± 1.1 | 9.9 ± 0.7 | 8.9 ± 0.5 | 9.7 ± 1.0 | F(1, 40) = 0.08, p = 0.77 |
| Cg1, 1.54 mm§ | 0.9 ± 0.1 | 1.1 ± 0.3 | 1.0 ± 0.2 | 1.1 ± 0.3 | 9.7 ± 0.7 | 9.0 ± 0.7 | 9.9 ± 0.6 | 9.5 ± 0.4 | F(1, 40) = 0.20, p = 0.65 |
| Cg2, 1.10 mm§ | 1.1 ± 0.2 | 1.5 ± 0.4 | 1.1 ± 0.3 | 1.4 ± 0.2 | 12.3 ± 1.0 | 11.4 ± 0.8 | 11.7 ± 0.6 | 11.4 ± 0.4 | F(1, 40) = 0.17, p = 0.68 |
| PrL, 1.98 mm§ | 2.8 ± 0.4 | 2.1 ± 04 | 2.7 ± 0.2 | 3.0 ± 0.4 | 12.4 ± 0.7 | 12.0 ± 0.7 | 11.1 ± 0.7 | 11.7 ± 0.5 | F(1, 40) = 0.02, p = 0.90 |
| PrL, 1.78 mm§ | 2.1 ± 0.3 | 2.1 ± 0.4 | 2.5 ± 0.3 | 2.3 ± 0.4 | 9.3 ± 0.8 | 10.7 ± 0.5 | 9.9 ± 0.4 | 9.8 ± 0.8 | F(1, 40) = 0.46, p = 0.50 |
| PrL, 1.54 mm§ | 2.1 ± 0.3 | 1.8 ± 0.4 | 2.1 ± 0.3 | 1.9 ± 0.2 | 10.9 ± 0.8 | 11.2 ± 0.7 | 10.9 ± 0.6 | 11.0 ± 0.5 | F(1, 40) = 0.03, p = 0.85 |
| IL, 1.98 mm§ | 2.3 ± 0.2‡‡ | 2.3 ± 0.1‡‡ | 2.4 ± 0.2‡‡ | 2.7 ± 0.1‡‡ | 5.0 ± 0.5 | 9.5 ± 0.5## | 9.4 ± 0.7** | 9.0 ± 0.7†† | F(1, 40) = 8.54, p < 0.01 |
| IL, 1.78 mm§ | 1.6 ± 0.3‡‡ | 2.3 ± 0.4‡‡ | 2.3 ± 0.2‡‡ | 1.9 ± 0.2‡‡ | 6.8 ± 0.4 | 12.2 ± 0.5## | 11.3 ± 0.4** | 11.5 ± 0.6†† | F(1, 40) = 9.10, p < 0.01 |
| IL, 1.54 mm§ | 1.6 ± 0.3‡‡ | 1.8 ± 0.4‡‡ | 1.5 ± 0.2‡‡ | 1.3 ± 0.3‡‡ | 6.0 ± 0.4 | 10.3 ± 0.6## | 10.1 ± 0.4** | 10.3 ± 0.6†† | F(1, 40) = 7.73, p < 0.01 |
| AI, 1.98 mm | No detectable expression | No detectable expression | |||||||
| AID, 1.78 mm | No detectable expression | No detectable expression | |||||||
| AID, 1.54 mm | No detectable expression | No detectable expression | |||||||
| AIV, 1.78 mm | No detectable expression | No detectable expression | |||||||
| AIV, 1.54 mm | No detectable expression | No detectable expression | |||||||
| PRh, −1.58 mm | No detectable expression | No detectable expression | |||||||
| Ect, −1.58 mm | No detectable expression | No detectable expression | |||||||
| Amygdala nuclei (all −1.58 mm) | |||||||||
| Lad§ | 1.2 ± 0.2 | 1.3 ± 0.1 | 1.4 ± 0.1 | 1.5 ± 0.1 | 2.3 ± 0.6 | 2.6 ± 0.4 | 2.6 ± 0.3 | 2.7 ± 0.3 | F(1, 40) = 0.06, p = 0.81 |
| Lav | No detectable expression | No detectable expression | |||||||
| BA§ | 3.6 ± 0.3 | 3.3 ± 0.3‡‡ | 3.3 ± 0.3‡‡ | 3.7 ± 0.3‡‡ | 2.5 ± 0.4 | 7.8 ± 0.9## | 7.6 ± 0.4** | 8.1 ± 0.8†† | F(1, 40) = 9.77, p < 0.01 |
| CeMa§ | 6.0 ± 0.7‡‡ | 6.8 ± 0.7‡ | 7.0 ± 1.2‡ | 7.5 ± 0.5‡ | 19.6 ± 1.0 | 9.9 ± 0.7## | 10.0 ± 0.8** | 10.8 ± 0.9†† | F(1, 40) = 15.33, p < 0.01 |
| CeLa§ | 7.3 ± 2.0 | 8.3 ± 1.6 | 7.8 ± 1.6 | 8.0 ± 1.9 | 15.4 ± 1.7 | 16.1 ± 1.1 | 15.1 ± 1.0 | 15.4 ± 1.0 | F(1, 40) = 0.01, p = 0.93 |
| CeCa§ | 6.5 ± 1.6 | 7.5 ± 1.5 | 7.3 ± 1.5 | 7.0 ± 1.6 | 10.8 ± 1.2 | 11.4 ± 0.9 | 10.1 ± 0.6 | 10.2 ± 1.1 | F(1, 40) = 0.03, p = 0.86 |
| Imp | No detectable expression | No detectable expression | |||||||
| Ilp | No detectable expression | No detectable expression | |||||||
| In | No detectable expression | No detectable expression | |||||||
| MePD§ | 2.8 ± 0.5 | 2.5 ± 0.4 | 3.1 ± 0.3 | 2.6 ± 0.5 | 5.6 ± 0.5 | 6.8 ± 0.8 | 6.4 ± 0.4 | 6.8 ± 0.5 | F(1, 40) = 0.12, p = 0.73 |
| MePV§ | 5.8 ± 0.5 | 5.3 ± 0.4 | 5.1 ± 0.7 | 6.0 ± 0.7 | 8.3 ± 0.5 | 8.3 ± 0.4 | 8.2 ± 0.3 | 8.3 ± 0.3 | F(1, 40) = 0.72, p = 0.40 |
| ACo§ | 5.8 ± 0.4 | 6.3 ± 0.4 | 5.5 ± 1.1 | 6.5 ± 0.5 | 10.6 ± 0.6 | 11.8 ± 0.5 | 9.6 ± 0.5 | 9.8 ± 0.5 | F(1, 40) = 0.63, p = 0.43 |
| PLCo§ | 5.5 ± 0.5 | 5.0 ± 0.3 | 5.3 ± 0.7 | 6.3 ± 0.4 | 8.1 ± 0.7 | 7.8 ± 0.7 | 7.4 ± 0.3 | 7.8 ± 0.6 | F(1, 40) = 0.18, p = 0.68 |
aWhole (sub)nucleus counted.
**p < 0.01, S1 control diet versus B6 control diet;
##p < 0.01, S1 control diet versus ZnR S1;
††p < 0.01, S1 control diet versus ZnR B6;
‡p < 0.05,
‡‡p < 0.01, within-strain and -diet conditioned versus unconditioned;
§p < 0.05, conditioned versus non-conditioned (significant effect of protocol).
Bold type indicates significant strain–diet–protocol ANOVA interactions. Data are mean ± SEM cell numbers. n = 8 per group for conditioned and n = 4 per group for unconditioned mice. ACo, Anterior cortical; AI, insular cortex; AIV, insular cortex, ventral; CeC, central, capsular; Cg1, cingulate area 1; Cg2, cingulate area 2; Ctl, control diet; Ect, ectorhinal cortex; M1, primary motor; M2, secondary motor; MePD, medial, posterodorsal; MePV, medial, posteroventral; PLCo, posterolateral cortical; PRh, perirhinal cortex.