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. Author manuscript; available in PMC: 2011 Aug 4.
Published in final edited form as: J Proteome Res. 2010 Jun 4;9(6):3073–3082. doi: 10.1021/pr901211j

Figure 1.

Figure 1

Separation, identification, and quantification of SILAC-labeled proteins. (A) Schematic flowchart for the discovery of molecular networks regulated by EGFR level in HNC using SILAC and LC–MS/MS. (B) SDS-PAGE separation of SILAC samples. S1 indicates the mixture of proteins from Lys0-labeled control cells and Lys8-labeled EGFR knockdown cells. S2 indicates the mixture of proteins from Lys0-labeled control cells and Lys8-labeled control cells. (C) Representative tandem mass spectrum for protein identification. (D) Representative SILAC pairs showing the regulation of proteins by EGFR knockdown.