Table 1.

Proteins Significantly Regulated by EGFR Knockdown

gene symbol	description	pep IDa	pep quantb	regc	siEGFR/CONd				blot?
					H/Le		H/Hf
					R1g	R2h	R1	R2
AARS	Alanyl-tRNA synthetase, cytoplasmic	14	6	↓	1.53	1.55	1.47	1.44
ACACA	Acetyl-CoA carboxylase 1	7	2	↓	1.29	1.50	1.44	1.42
ACADVL	Very long-chain specific acyl-CoA dehydrogenase	18	4	↑	1.62	1.64	1.46	1.49
ACAT2	Acetyl-CoA acetyltransferase, cytosolic	10	5	↓	1.45	1.47	1.40	1.43
ACLY	ATP-citrate synthase	27	14	↓	1.39	1.42	1.43	1.41
ALDH1A3	Aldehyde dehydrogenase family 1 member A3	22	8	↓	1.88	1.84	1.46	1.58	Yes
ANK3	Ankyrin-3 (Ankyrin-G)	6	2	↑	2.37	2.69	1.71	1.92
CDC2	Cell division control protein 2 homologue	12	6	↓	2.85	2.76	2.20	2.28
CDKN1A	cyclin-dependent kinase inhibitor 1 (p21)	2	1	↑	1.56	1.62	1.43	1.46
EGFR	Epidermal growth factor receptor	42	20	↓	2.05	2.28	2.42	2.51	Yes
FABP5	Fatty acid-binding protein 5	7	3	↑	2.07	2.17	1.68	1.48
FASN	Fatty acid synthase	54	53	↓	1.37	1.36	1.50	1.49
FDFT1	Squalene synthetase	8	2	↓	1.47	1.74	1.50	1.73
FDPS	Farnesyl pyrophosphate synthetase	8	2	↓	1.61	1.48	1.87	1.59
GLUL	Glutamine synthetase	5	2	↓	1.95	1.75	1.57	1.50
HADHA	Trifunctional enzyme subunit alpha, mitochondrial	24	10	↑	1.63	1.62	1.48	1.44
HADHB	Trifunctional enzyme subunit beta, mitochondrial	14	4	↑	1.65	1.74	1.44	1.49
HMGCS1	Hydroxymethylglutaryl-CoA synthase, cytoplasmic	12	7	↓	2.26	2.48	2.04	2.03
IRF6	Interferon regulatory factor 6	7	4	↑	2.40	2.36	1.58	1.67
KRT17	Keratin 17	23	12	↑	2.52	3.08	1.41	1.42
LGALS7	Galectin-7	8	4	↑	5.44	5.42	1.41	1.41	Yes
MAT2A	Methionine adenosyltransferase IIα	8	2	↓	1.92	1.43	1.88	1.45	Yes
MCM2	DNA replication licensing factor MCM2	6	2	↓	2.08	2.18	1.82	2.79
MCM6	DNA replication licensing factor MCM6	5	2	↓	2.25	2.34	1.70	2.50
MTA2	Metastasis-associated protein MTA2	5	1	↓	1.57	1.39	1.81	1.68
PCNA	Proliferating cell nuclear antigen	11	6	↓	1.96	1.91	1.52	1.69
PHLDA1	Pleckstrin homology-like domain family A member 1	3	2	↓	2.05	1.71	1.50	1.44
PHLDA2	Pleckstrin homology-like domain family A member 2	11	3	↓	2.10	2.06	2.09	2.16
PHLDA3	Pleckstrin homology-like domain family A member 3	9	3	↓	1.06	1.13	1.40	1.46
PTGES3	Prostaglandin E synthase 3	2	1	↓	1.46	1.52	1.42	1.69
RFC5	Replication factor C subunit 5	2	1	↓	1.61	1.73	1.42	1.51
SERPINB5	Serpin B5 (Maspin)	6	4	↑	1.97	1.91	1.52	1.43
SFN	14–3–3 sigma (Stratifin)	23	16	↑	1.95	1.94	1.46	1.45
TK1	Thymidine kinase, cytosolic	2	2	↓	n/ai	4.31	n/a	3.19	Yes
TPPP3	Tubulin polymerization-promoting protein family member 3	3	2	↑	3.99	4.06	1.61	1.62
VCL	Vinculin	33	16	↓	1.55	1.49	1.56	1.42

^aUnique peptide identified.

^bPeptide quantitated.

^cRegulation.

^dNormalized siEGFR/CON fold change.

^e“Heavy”/“Light”.

^f“Heavy”/“Heavy”.

^gReplicate 1.

^hReplicate 2.

ⁱNot applicable.