Table 1. ABPP-MudPIT analysis of serine hydrolase activities in human prostate cancer cell lines.
Serine hydrolase activities were filtered for enzymes that displayed: 1) > 10-fold higher spectral counts in FP-biotin-treated proteomes compared to "no-probe" control proteomes for at least one of the cancer cell lines examined, and 2) an average of ≥ 6 spectral counts for at least one of the cancer cell lines examined. Data represent average values ± SEM for 3–4 independent experiments per cell line. See also Table S1.
| LNCaP |
PC3 |
DU145 |
|||||
|---|---|---|---|---|---|---|---|
| Protein | abbreviation | av | sem | av | Sem | av | sem |
| prolyl endopeptidase | PREP | 455 | 64 | 423 | 144 | 132 | 11 |
| FASN Fatty acid synthase | FASN | 1850 | 135 | 1324 | 111 | 1385 | 170 |
| Acyl-protein thioesterase 1 | LYPLA1* | 81 | 5 | 21 | 8 | 55 | 1 |
| Abhydrolase domain-containing protein 10 | ABHD10 | 622 | 132 | 534 | 111 | 252 | 29 |
| Acylamino-acid-releasing enzyme | APEH | 571 | 45 | 642 | 88 | 196 | 32 |
| Probable serine carboxypeptidase CPVL | CPVL* | 0 | 0 | 27 | 4 | 6 | 1 |
| Platelet-activating factor acetylhydrolase 2 | PAFAH2 | 19 | 6 | 22 | 4 | 25 | 4 |
| prolyl endopeptidase-like isoform C | PREPL | 63 | 13 | 98 | 12 | 145 | 25 |
| Acyl-protein thioesterase 2 | LYPLA2* | 31 | 15 | 138 | 7 | 106 | 13 |
| platelet-activating factor acetylhydrolase IB subunit gamma | PAFAH1B3 | 72 | 13 | 0 | 0 | 43 | 17 |
| Dipeptidyl peptidase 9 | DPP9* | 50 | 8 | 445 | 45 | 244 | 24 |
| Patatin-like phospholipase domain-containing protein 4 | PNPLA4 | 49 | 14 | 42 | 3 | 15 | 3 |
| Protein phosphatase methylesterase 1 | PPME1 | 63 | 5 | 48 | 12 | 14 | 3 |
| palmitoyl-protein thioesterase 2 | PPT2* | 3 | 2 | 22 | 8 | 42 | 5 |
| Uncharacterized protein ABHD12 | ABHD12 | 109 | 23 | 107 | 19 | 107 | 19 |
| Platelet-activating factor acetylhydrolase IB | PAFAH1B2 | 60 | 23 | 101 | 14 | 66 | 31 |
| Abhydrolase domain-containing protein 11 | ABHD11* | 258 | 26 | 136 | 17 | 89 | 17 |
| Retinoid-inducible serine carboxypeptidase | SCPEP1 | 121 | 33 | 47 | 14 | 63 | 5 |
| arylacetamide deacetylase-like 1 (KIAA1363) | AADACL1* | 58 | 15 | 373 | 59 | 593 | 107 |
| Abhydrolase domain-containing protein 6 | ABHD6 | 55 | 11 | 62 | 9 | 81 | 11 |
| Tripeptidyl peptidase II | TPP2 | 4 | 2 | 10 | 1 | 3 | 1 |
| prolylcarboxypeptidase | PRCP | 77 | 13 | 49 | 27 | 33 | 9 |
| Retinoblastoma-binding protein 9 | RBB9 | 9 | 5 | 10 | 3 | 1 | 0 |
| Dipeptidyl peptidase 8 | DPP8 | 9 | 3 | 16 | 3 | 23 | 11 |
| cathepsin A | CTSA | 12 | 5 | 8 | 0 | 17 | 2 |
| Abhydrolase domain-containing protein FAM108A1 | FAM108A1 | 7 | 3 | 8 | 2 | 31 | 9 |
| Neuropathy target esterase | PNPLA6* | 46 | 11 | 211 | 27 | 106 | 22 |
| Lysophospholipase-like protein 1 | LYPLAL1 | 73 | 21 | 25 | 11 | 27 | 7 |
| Presenilins-associated rhomboid-like protein | PARL | 8 | 3 | 8 | 1 | 9 | 2 |
| Abhydrolase domain-containing protein FAM108B1 | FAM108B1 | 12 | 6 | 31 | 10 | 51 | 9 |
| BAT5 | BAT5 | 28 | 7 | 20 | 3 | 20 | 3 |
| Abhydrolase domain-containing protein 3 | ABHD3 | 2 | 1 | 7 | 2 | 9 | 4 |
| Sialate O-acetylesterase | SIAE* | 94 | 20 | 10 | 1 | 7 | 0 |
| Isoamyl acetate-hydrolyzing esterase 1 | IAH1 | 2 | 1 | 5 | 1 | 1 | 1 |
| Sn1-specific diacylglycerol lipase beta | DAGLB | 6 | 2 | 20 | 1 | 10 | 1 |
| monoglyceride lipase (MAGL) | MGLL* | 11 | 2 | 54 | 14 | 116 | 24 |
| carboxylesterase 2 | CES2 | 18 | 4 | 9 | 2 | 10 | 3 |
| PNPLA8 89 kDa protein | PNPLA8 | 15 | 3 | 11 | 2 | 2 | 0 |
| Abhydrolase domain-containing protein 4 | ABHD4 | 27 | 7 | 5 | 1 | 6 | 1 |
| btyrylcholinesterase | BCHE | 10 | 4 | 0 | 0 | 0 | 0 |
| Uncharacterized protein LACTB | LACTB | 2 | 1 | 2 | 1 | 5 | 1 |
| Lon protease | LONP1 | 48 | 4 | 34 | 9 | 14 | 2 |
| Fatty-acid amide hydrolase 1 | FAAH* | 181 | 56 | 0 | 0 | 9 | 2 |
| urokinase plasminogen activator | PLAU | 0 | 0 | 1 | 0 | 16 | 4 |
| similar to Dipeptidyl-peptidase 2 precursor | DPP7* | 170 | 48 | 14 | 1 | 8 | 2 |
| Dipeptidyl peptidase 4 | DPP4 | 38 | 7 | 89 | 9 | 1 | 0 |
| acetylcholinesterase | ACHE | 0 | 0 | 4 | 1 | 1 | 1 |
| Abhydrolase domain-containing protein 2 | ABHD2 | 22 | 7 | 0 | 0 | 0 | 0 |
| Fatty-acid amide hydrolase 2 | FAAH2 | 6 | 2 | 0 | 0 | 0 | 0 |
| Isoform 1 of Tissue-type plasminogen activator | PLA | 0 | 0 | 9 | 2 | 0 | 0 |
*
denotes serine hydrolase activities that were significantly higher (highlighted in red) or lower (highlighted in blue) in both PC3 and DU145 cells versus LNCaP cells (i.e. p<0.01 for PC3 versus LNCaP and DU145 versus LNCaP)