Table 3.
Gene copy number by qPCR
GENE | MET | RON | HER2 | FGFR2 | MSP | HGF | SMAD4 | |
Chromosome | 7q31 | 3p21.3 | 17q21.1 | 10q26.1 | 3p21 | 7q21.1 | 18q21.1 | |
Gastric cell lines | ||||||||
HS746t | 9.51 | 2.78 | 1.88 | 3.42 | 1.68 | 1.03 | ||
KatoIII | 0.89 | 2.32 | 3.10 | 486.00 | 3.19 | 0.68 | 2.20 | |
NCI | 0.87 | 1.08 | 114.14 | 0.83 | 1.15 | 0.86 | ||
AGS | 1.69 | 2.37 | 1.91 | 2.41 | 1.28 | 0.84 | ||
MKN-45 | 17.60 | 2.20 | 2.59 | 1.78 | 1.91 | 0.07 | ||
Snu-1 | 1.65 | 3.04 | 2.25 | 3.14 | 2.07 | 2.30 | ||
Snu-5 | 18.33 | 2.28 | 1.95 | 3.40 | 7.22 | 0.84 | ||
Snu-16 | 2.49 | 2.08 | 1.77 | 320.00 | 2.53 | 3.86 | 2.19 | |
Patient | Frozen tissues (OCT) | |||||||
sample # | ||||||||
1 | F2D | 3.61 | 1.70 | 1.86 | 2.46 | 2.09 | 2.48 | 0.80 |
2 | F3D (adjacent nl of F4) | 2.04 | 1.605 | |||||
2 | F4D | 4.28 | 1.19 | 42.98 | 1.64 | 2.19 | 2.61 | 1.18 |
3 | F6D | 2.22 | 1.36 | 1.50 | 2.74 | 1.71 | 1.85 | 1.28 |
4 | F8D | 2.16 | 1.74 | 1.52 | 1.42 | 1.92 | 2.32 | 1.00 |
5 | F10D | 2.21 | 1.60 | 1.52 | 2.11 | 1.25 | 1.67 | 1.18 |
6 | F11D | 2.01 | 2.41 | 1.48 | 1.85 | 1.86 | 1.77 | 0.87 |
7 | F14D | 1.83 | 1.05 | 1.31 | 1.47 | 1.64 | 2.17 | 0.45 |
8 | F15D | 1.91 | 1.59 | 1.58 | 1.63 | 2.39 | 1.87 | 1.09 |
9 | F18D | 2.94 | 1.43 | 2.02 | 1.72 | 1.82 | 2.74 | 1.31 |
10 | F23 gastritis | 1.91 | 0.65 | |||||
11 | F24 gastritis | 2.93 | 0.99 | |||||
12 | F25 gastritis | 1.99 | 0.53 | |||||
13 | F26 gastritis | 2.15 | 0.61 | |||||
Patient | Paraffin embedded (FFPE) | |||||||
sample # | ||||||||
1 | P1D LN | 2.38 | 3.6 | 1.51 | 2.4 | 1.82 | ||
1 | P2D primary | 1.44 | 4.01 | 2.93 | 2.78 | 3.33 | 0.91 | 2.24 |
2 | P3D | 2.46 | 4.49 | 3.97 | 2.27 | 1.97 | 1.92 | |
3 | P4D LN | 2.29 | 4.1 | 3.37 | 1.4 | 3.25 | 2.56 | 1.88 |
3 | P5D primary | 1.85 | 2.97 | 2.47 | 0.42 | |||
4 | P6D | 2.34 | 6.29 | 3.54 | 0.99 | 3.4 | 1.45 | 1.83 |
5 | P7D | 2.05 | 3.15 | 2.79 | 1.89 | 1.83 | 1.35 | 2.19 |
6 | P8D | 6.17 | 24.3 | 8.77 | 1.77 | 9.63 | 3.39 | 2.7 |
7 | P9D primary | 74.3 | 38.29 | 16.98 | 6.2 | 17.86 | 2.8 | 3.89 |
7 | P10D LN | 21.77 | 43.83 | 55.69 | 10.83 | 0.98 | 14.48 | |
8 | P11D LN | 3.07 | 3.9 | 4.8 | 0.58 | 3.06 | 2.38 | 3.4 |
8 | P12D primary | 2.87 | 5.19 | 2.56 | 3.88 | |||
9 | P13D primary | 4.04 | 9.1 | 0.95 | 7.61 | 1.9 | ||
9 | P14D LN | 2.9 | 11.97 | 49.4 | 0.45 | 6.1 | 1.51 | 4.94 |
10 | P15D | 2.11 | 7.3 | 6.03 | 3.02 | 4.78 | 1.85 | 3.86 |
11 | P16D | 2.29 | 4.07 | 3.12 | 0.63 | 4.24 | 1.24 | 3.02 |
12 | P17D primary | 1.43 | 7.6 | 7.78 | 1.15 | 3.99 | 1.26 | 2.01 |
12 | P18D LN | 2.19 | 4.29 | 2.98 | 1.62 | |||
13 | P19D | 3.09 | 5.46 | 3.51 | 0.69 | 4.41 | 2.65 | 4.56 |
14 | P20D primary | 1.83 | 3.43 | 0.7 | 3.31 | 0.69 | ||
14 | P21D LN | 2.46 | 4.43 | 2.76 | 3.73 | 1.85 | 3.25 | |
15 | P22D primary | 2.8 | 5.55 | 0.8 | 4.15 | 1.28 | ||
15 | P23D LN | 2.44 | 13.06 | 4.89 | 5.31 | 1.17 | 4.72 | |
16 | P24D LN | 2.1 | 4.11 | 4.57 | 3 | 1.35 | 4.43 | |
16 | P25D | 1.98 | 3.52 | 0.86 | 3.11 | 1.1 | ||
17 | P26D | 10.73 | 115.1 | 39.26 | 11.41 | 92.7 | 4.56 | 13.5 |
18 | P27D primary | 2.74 | 5.36 | 4.78 | 0.59 | 4.09 | 1.35 | 3.66 |
18 | P28D LN | 2.16 | 4.29 | 2.39 | 1.1 | |||
19 | P29D primary | 1.73 | 2.63 | 1.06 | 2.65 | 1.47 | ||
19 | P30D LN | 1.97 | 5.36 | 16.4 | 4.28 | 0.99 | 3.27 | |
20 | P31D | 1.87 | 3.93 | 3.01 | 0.82 | 2.6 | 1.1 | 2.39 |
21 | P32D | 4.14 | 7.61 | 4.17 | 0.65 | 6.46 | 1.01 | 3.08 |
22 | P33D | 1.81 | 6.97 | 4.64 | 0.91 | 4.68 | 1.15 | 2.8 |
23 | P34 primary | 3.57 | 5.91 | 4.66 | 5.19 | 1.9 | 5.38 | |
23 | P35 liver met | 2.25 | 15.6 | 2.39 | 8.49 | 1.08 | ||
23 | P36D LN | 3.34 | 4.78 | 7.2 | 1.44 | |||
24 | P37 LN | 3.04 | 7.25 | 6.98 | 5.16 | 1.68 | 6.68 | |
24 | P38D primary | 2.07 | 2.73 | 2.23 | 1.08 | |||
25 | P39D primary | 5.62 | 32.59 | 5.97 | 17.59 | 2.51 | ||
25 | P40D LN | 3.48 | 24.79 | 29.4 | 15.85 | 1.33 | 18.66 | |
26 | P41D | 4.05 | 7.73 | 16.87 | 0.74 | 8.07 | 1.32 | 13.54 |
27 | P42D | 3.08 | 6.76 | 6.67 | 3.74 | 1.36 | 4.08 | |
28 | P43D | 0.44 | 32.2 | 105.28 | 21.36 | 17.49 | 0.42 | 32.19 |
29 | P44D primary | 2.73 | 14.13 | 10.97 | 1.46 | 5.06 | 0.5 | 7.2 |
29 | P45 LN | 3.3 | 8.2 | 5.45 | 1.52 | |||
30 | P46 | 8.91 | 79.19 | 151.33 | 7.5 | 26.48 | 1.01 | 73.6 |
31 | P49 | 1.81 | 1.75 | 2.09 | 0.65 | 2.13 | 1.21 | 2.04 |
32 | P52 | 2.45 | 3.18 | 4.92 | 0.59 | 2.53 | 1.48 | 4.7 |
33 | P53 | 2.09 | 2.81 | 3.35 | 0.92 | 2.38 | 1.95 | 2.93 |
34 | P56 recur | 2.11 | 3.55 | 2.79 | 0.8 | 3.46 | 1.16 | 2.88 |
34 | P59 2000 | 3.23 | 3.25 | 2.45 | 1.4 | |||
35 | P61 | 3.22 | 3.08 | 3.22 | 0.87 | 2.36 | 1.32 | 3 |
36 | P65 gallbladder met | 2.42 | 2.7 | 2.38 | 0.79 | 2.05 | 1.54 | 2.52 |
36 | P67 primary | 1.91 | 1.43 | 0.8 | 1.71 | |||
Adjacent normals (FFPE) | ||||||||
6 | P68 nl stomach of P8 | 3.06 | 1.7 | |||||
7 | P69 nl prox mg P9,10 | 4.75 | 2.53 | |||||
9 | P70 nl antrum of P13,14 | 1.84 | 2.35 | 1.03 | 2.32 | |||
24 | p71 nl distal mg of P37,38 | 3.05 | 1.98 | |||||
29 | p72 nl stomach P44,45 | 3.87 | 2.65 | |||||
Esophageal cell lines | ||||||||
TE1 | 2.08 | |||||||
TE8 | 1.92 | |||||||
TE12 | 1.78 | |||||||
SGKT4 | 2.4 | |||||||
SGKT5 | 3.28 | |||||||
Flo | 2.53 | |||||||
KYSE 110 | 3.11 | |||||||
KYSE 140 | 2.54 | |||||||
KYSE 220 | 2.59 | |||||||
KYSE 520 | 2.98 | |||||||
KYSE 550 | 2.44 |