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. 2011 May-Jun;2(3):189–194. doi: 10.4161/nucl.2.3.16246

Figure 3.

Figure 3

Schematic illustration of the traffic of nucleolar processing proteins during nucleolar assembly in incipient nucleoli. The traffic of processing proteins tagged with photoactivatable green fluorescent proteins (PAGFP) activated by laser (PA) was analyzed using fibrillarin for DFC marker and using PAGFP-Nop52 for GC marker. The pool of PAGFP-fibrillarin activated in one incipient nucleolus (green) migrates to the other incipient nucleoli but not to PNBs. The pool of PAGFP-Nop52 activated in one incipient nucleolus (green) migrates to the other incipient nucleoli and also to PNBs. This explains the progressive formation of GC controlled by PNBs as described in reference 42.