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. 2011 May-Jun;2(3):208–218. doi: 10.4161/nucl.2.3.15736

Figure 5.

Figure 5

Differential co-localization of molecular partners in pre-I structures. Cells expressing YFP-Ala10-PABPN1 (Ala10) or YFP-Ala16-PABPN1 (Ala16) were fixed 30 hours post transfection and were immunolabeled for PAP, Hsp70 and poly-ubiquitin. PAP and ubiquitin antibodies were visualized with an Alexa 594 conjugated secondary antibody and the Hsp70 antibody was recognized with a DEAC-conjugated secondary antibody. Co-localization between PABPN1 and the antibody is visualized in the merged images. Scale bars are 5 µm.