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. 2011 May-Jun;2(3):229–245. doi: 10.4161/nucl.2.3.15893

Figure 7.

Figure 7

Nucleolar substructure and cell proliferation change under NOL7 RNAi conditions. NOL7 siRNA treatment was performed and the following endogenous proteins were stained: (A) NOL7 and fibrillarin. (B) Fibrillarin and UBF. (C) Fibrillarin and B23. (D) Counting of number of nucleoli per cell before and after siRNA. (E) Proliferation of cells is reduced in siRNA treated cells as measured by the MTT assay for measuring cell viability (at 72 hrs) or cell counting (24, 48, 72 and 96 hrs). Standard deviation from n = 6 different plates. Bar, 10 µm.