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. Author manuscript; available in PMC: 2011 Aug 4.
Published in final edited form as: Cancer Res. 2008 Oct 15;68(20):8564–8572. doi: 10.1158/0008-5472.CAN-08-2232

Figure 4.

Figure 4

Effect of delphinidin on phospho-NF-κB/p50 and p65 and NF-κB DNA binding activity in PC3 cells. Nuclear extracts were prepared from cells treated with vehicle alone (0.1% DMSO) and specified concentrations of delphinidin in 0.1% DMSO for 48 h. A, protein levels of phospho-NF-κB/p50(ser529) and phospho-NF-κB/p65(ser536) in nuclear lysates of PC3 cells as determined by immunoblot analysis. Equal loading of protein was assessed by stripping and reprobing the blots with lamin antibody. B, NF-κB DNA binding activity in PC3 cells as detailed in Materials and Methods. Lane 1, NF-κB-DNA complex. Lane 2, the shift in NF-κB-DNA complex due to binding with NF-κB/p65 specific antibody. Lanes 3 to 5, change in the NF-κB/p65 oligo-immune complex due to treatment with delphinidin. C, immunocytochemistry of NF-κB/p65 in PC3 cells. D, specific ELISA for NF-κB/p65 was done in nuclear lysates of PC3 cells treated with vehicle alone (0.1% DMSO) and specified concentrations of delphinidin.