Compounds selected from the Maybridge HitFinder library: their p53 activity, cytotoxicity, and caspase 3/7 activity. Maybridge HitFinder library was screened by high throughput assays for p53 activity, caspase 3/7 and MAGE specific cytotoxicity in p53RE-bla HCT-116 cell line, as described in Materials and methods section. Twenty-two compounds scored high on these criteria and eight compounds were further selected based on the highest p53 activity (RFU of 1.0 or higher) and no or low caspase 3/7 activity (2.5 RLU or lower). p53 activity, cytotoxicity and caspase activation of these eight compounds is demonstrated in this figure 1. As shown in the figure, three of the compounds, HTS11125, GK02432 and SPB02270, show highest p53 activity (highlighted in red).