Figure 4. The illustrated thresholds from four statistical methodologies overlaid onto the associated genomic population.

For (a, b, c, d): genomic screen 1 (GS1), genomic screen 2 (GS2), sum rank (SR), median absolute deviation (MAD), z-score, and strictly standardized mean difference (SSMD). a, Each point (▪) represents the % Infection for the analyzed population in GS1^AB plotted against the % Infection from the corresponding wells within GS1^CD. The illustrated lines indicate the thresholds within which hits are declared if SR ≤ 0.001350, MAD ≤ −2.000, z-score ≤ −3.000 or SSMD ≤ −3.000. b, Similar to a, excepting that the % Infection from GS2^AB is plotted against the % Infection for corresponding wells from GS2^CD. The thresholds indicate the limits within which hits can be declared assuming SR ≤ 0.001350, MAD ≤ −3.000, z-score ≤ −3.000 or SSMD ≤ −3.000. c, The thresholds for the top 200 (dashed lines) and top 500 (solid lines) lists generated by SR, MAD, z-score and SSMD are overlaid onto the scatter plot of the % Infection (as described in a) for the paired siRNA pools in GS1^AB and GS1^CD. d, The % Infection for corresponding siRNA pools within GS2 are plotted as coordinates (GS2^AB, GS2^CD). The limits determining the top 200 (dashed lines) and top 500 (solid lines) lists are illustrated in the context of the GS2 population.