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. Author manuscript; available in PMC: 2011 Nov 14.
Published in final edited form as: J Mol Biol. 2010 Nov 5;405(2):410–426. doi: 10.1016/j.jmb.2010.10.040

Fig. 5.

Fig. 5

Optimization of gene transfer. (a) Bivalent targeting adapters enable improved Ad transduction of Her2-expressing cells. V, virus alone; MVA, monovalent 2E6-G5 adapter, virus in complex with the control monovalent adapter 2E6-G5; L1-L6, virus in complex with one of the six bivalent and bispecific adapters (2E6-Ln-2E6-L3-G5) each containing two copies of 2E6 connected with linkers of various length. L1 denotes Gly4Ser, L2 denotes (Gly4Ser)2, etc. Adapter-to-fiber ratios are shown below the graph. (b) Increased valency of targeting adapter yields improved transduction of Her2-expressing cells. Transgene expression by the vector alone (V) or by the vector in a complex with mono- (1), bi- (2) or trivalent (3) adapters in which the 2E6 DARPin modules are spaced by a (Gly4Ser)2 linker. Transgene expression by CAR binding-ablated Ad in cultured cells is shown as activity of luciferase reporter in the lysates of infected cells displayed in relative light units. Error bars show standard deviations calculated for triplicate data points.