Figure 6.
Stability of TPK1-GFP and posttranslational release of free GFP. Protoplasts prepared from wild-type or transgenic (TPK1-GFP) Arabidopsis leaves were pulse labeled for 1 h with a mixture of 35S-Met/Cys and subjected to chase for the indicated hours. Homogenates were immunoselected with anti-GFP antiserum and analyzed by SDS-PAGE and fluorography. B is from a shorter gel exposure of the gel in A. The positions of TPK1-GFP (black arrowhead), free GFP (white arrowhead), and 20-kD polypeptide transiently associated to TPK1-GFP (asterisk) are indicated at right. Numbers at left indicate the positions of molecular mass markers, in kD.