Figure 4.

In vitro binding of STM to the CUC1 promoter. A, Scheme representing the CUC1 promoter. Putative STM-binding sites identified by SELEX are highlighted with blue squares and the sequence identified to be overrepresented in STM-VP16 induced genes (Supplemental Table S5) is indicated with a dashed line. B and C, EMSA with CUC1 promoter using recombinant STM homeodomain (B) or whole protein (C). D, One-hybrid experiment in yeast using wild-type and mutated CUC1 promoter. Growth in the absence of His due to activation of the HIS3 gene under the control of the CUC1 promoter was monitored using serial dilutions of the corresponding yeast strains. 3AT, 3-Amino-1,2,4-triazole. [See online article for color version of this figure.]