Figure 6.

RNAi suppression of APY1 in an apy2 single knockout results in increased stomatal apertures compared with the Ws wild type (WSWT). A, Treatments with light and 10 μm ABA induce more open stomata in leaves of RNAi plants treated with estradiol compared with leaves of Ws wild-type plants treated with estradiol (1.5 μm average width for control). B, Treatments with light and 10 μm ABA have no effect on stomatal apertures in non-estradiol-treated leaves of RNAi plants compared with leaves of non-estradiol-treated Ws wild-type plants (1.7 μm average width for control). Apertures were measured as width/length after 2 h of treatment. Error bars represent se. Different letters above the bars indicate mean values that are significantly different from one another as determined by Student’s t test (P < 0.05; n ≥ 50). These data are representative of three or more biological repeats.