Figure 3.

Relationship of pathogen-induced zealexin concentrations with Tps6 and Tps11 transcript levels. Average (n = 4; ±sem). A, Zealexin A1. B, Zealexin A3 levels in maize stems experiencing no treatment (○), damage plus water (•), or damage plus water suspensions of R. microsporus (▾, 10⁷ spores mL⁻¹), A. flavus (△, 10⁷ spores mL⁻¹), or F. graminearum (10⁶ spores mL⁻¹) at time 0 or 4, 12- and 24-h postinoculation. C, Tps6. D, Tps11 corresponding average (n = 4; ±sem) qRT-PCR fold change of transcripts as compared to levels of Tps6 in control stems. E, Tps6. F, Tps11 relationship between fungal-induced fold change in gene expression and total zealexins at 24 h. Within plots, different letters (a–d) represent significant differences at 24 h (all ANOVAs, P < 0.0001; Tukey test corrections for multiple comparisons, P < 0.05).