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. 2011 Jun 17;156(4):1772–1782. doi: 10.1104/pp.111.180208

Figure 2.

Figure 2.

Analysis of transgenic bbx24-1 lines expressing EGFP-bbx24NLS. A, Five-day-old dark-grown wild-type or bbx24-1 seedlings overexpressing EGFP-BBX24, and transgenic bbx24-1 seedlings overexpressing EGFP-bbx24NLS were analyzed by fluorescence microscopy before (Dark), and after being transferred to cWL at the indicated time points. Images taken from hypocotyls are shown. Bar = 50 μm. Inserted sections are single-cell images analyzed by CLSM. Bar = 10 μm. B, Western-blot analysis of similarly treated plant material for the detection of EGFP-BBX24 or EGFP-bbx24NLS fusions. Antibodies against BBX24 or actin were used for the immune detection. C, Hypocotyl-length measurements of 5-d-old wild-type, bbx24-1, and bbx24-1 overexpressing EGFP-BBX24 or EGFP-bbx24NLS, grown under different fluence rates of cR, cFR, and cB light.