Table V. 14Redistribution of radiolabel after incubation with [U-14C]Glc in tuber discs of B33-TPS and B33-TPP lines.
Tuber discs were cut from growing tubers of six separate plants per genotype. After 4 h of incubation, the tuber discs were extracted and fractionated. Values represent means ± sd of measurements of six individual plants per genotype. Values in boldface denote fractions further fractionated into subfractions. Values in italics show significant subfractions of the soluble fraction. * Significant difference (P < 0.05) according to Student’s t test (II).
| Parameter | 14C Incorporation | |||
| Wild Type | B33-TPS 16 | B33-TPS 17 | B33-TPS 29 | |
| Total metabolized (Bq g−1 fresh wt) | 1,468.4 ± 111.7 | 1,649.6 ± 330.0 | 1,460.3 ± 329.0 | 1,431.6 ± 176.8 |
| Insoluble (%) | 30.8 ± 6.1 | 38.1 ± 7.1 | 22.7 ± 5.8 | 36.9 ± 10.1 |
| Protein (%) | 1.8 ± 0.5 | 2.7 ± 0.7 | 3.2 ± 1.3 | 2.9 ± 0.7 |
| Starch (%) | 17.9 ± 6.8 | 22.3 ± 6.9 | 9.5 ± 5.5 | 20.1 ± 8.6 |
| Cell wall (%) | 11.1 ± 1.4 | 13.1 ± 2.9 | 10.0 ± 3.1 | 14.0 ± 4.3 |
| Soluble (%) | 69.2 ± 6.1 | 61.9 ± 7.1 | 77.3 ± 5.8 | 63.1 ± 10.1 |
| Amino acid (%) | 3.8 ± 0.7 | 4.3 ± 0.9 | 4.2 ± 1.2 | 3.8 ± 0.3 |
| Sugars (%) | 57.5 ± 7.4 | 46.2 ± 8.1 | 59.8 ± 5.7 | 45.1 ± 9.8 |
| Acidic (%) | 7.9 ± 1.2 | 11.3 ± 1.6* | 13.2 ± 5.4 | 14.1 ± 1.3* |
| Organic acids (%) | 5.4 ± 1.0 | 8.9 ± 1.1* | 10.1 ± 4.1* | 10.7 ± 1.2* |
| Released CO2 (%) | 2.7 ± 0.2 | 3.6 ± 0.7* | 4.0 ± 0.8* | 4.0 ± 0.5* |
| Parameter | 14C Incorporation | |||
| Wild Type | B33-TPP 11 | B33-TPP 26 | B33-TPS 34 | |
| Total metabolized (Bq g−1 fresh wt) | 631.8 ± 179.1 | 733 ± 111.1 | 666.2 ± 188.9 | 445.9 ± 55.5 |
| Insoluble (%) | 46.3 ± 21.5 | 48.1 ± 19.7 | 39.1 ± 13.6 | 36.9 ± 8.5 |
| Protein (%) | 2.2 ± 0.8 | 1.9 ± 0.9 | 1.9 ± 0.5 | 1.9 ± 1.2 |
| Starch (%) | 32.7 ± 16.6 | 33.9 ± 14.7 | 27.2 ± 12.1 | 25.5 ± 6.5 |
| Cell wall (%) | 11.4 ± 4.8 | 12.5 ± 4.9 | 10.0 ± 2.1 | 9.5 ± 2.9 |
| Soluble (%) | 37.2 ± 10.0 | 37.8 ± 13.7 | 34.6 ± 9.4 | 44.1 ± 24.5 |
| Amino acid (%) | 12.9 ± 6.8 | 13.0 ± 3.2 | 15.3 ± 2.2 | 11.9 ± 3.6 |
| Acidic (%) | 12.2 ± 6.0 | 9.5 ± 5.5 | 6.7 ± 5.9 | 11.2 ± 6.2 |
| Organic acids (%) | 11.7 ± 1.0 | 12.1 ± 1.2 | 10.9 ± 0.4 | 11.1 ± 0.7 |
| Released CO2 (%) | 3.2 ± 0.9 | 2.4 ± 0.3 | 3.0 ± 1.1 | 3.4 ± 1.1 |
| Suc (%) | 6.7 ± 1.3 | 23.6 ± 6.3* | 17.6 ± 4.4* | 25.6 ± 1.8* |
| Other sugars (%) | 6.3 ± 1.5 | 9.0 ± 3.7* | 9.8 ± 1.0* | 5.2 ± 1.9 |