Figure 9.

CaRING1-OX transgenic Arabidopsis plants show enhanced resistance to H. arabidopsidis isolate Noco2 infection. A, Disease symptoms and aniline blue-stained mycelia on cotyledons of wild-type (WT) and transgenic Arabidopsis plants at 7 d and 2 to 3 d after spray inoculation with H. arabidopsidis isolate Noco2 (5 × 10⁴ spores mL⁻¹), respectively. B, Production of sporangiophores per cotyledon of wild-type and transgenic plants 6 d after inoculation with H. arabidopsidis. Single cotyledons from over 60 seedlings per genotype were analyzed with the microscope and categorized into one of five categories of sporangiophore production (0–5, 6–10, 11–15, 16–20, or >20, depending on the number of sporangiophores observed). Average numbers of newly formed sporangiophores on cotyledons of wild-type and transgenic lines are indicated below each of the lines analyzed. C, Sporulation of H. arabidopsidis isolate Noco2 per cotyledon at 8 d after inoculation. Each experiment contained average spore counts from 50 inoculated cotyledons of wild-type and transgenic lines. Statistically significant differences between means were determined using Fisher’s lsd test (P < 0.05). [See online article for color version of this figure.]