Table I. Sugar composition and macromolecular characteristics of wild-type, cesa5-1, and mum5-1 water-extracted mucilage.
Values in parentheses are sd of four independently extracted samples from two biological repeats. Mn, Number-average molar mass; Mp, molar mass at peak maximum; Mw, weight-average molar mass; I, polydispersity index; [η], average intrinsic viscosity; a, Mark-Houwink coefficient; –, not determined.
| Measurement | Wild-Type Total Water-Extracted Mucilage | Wild-Type Polymeric Fraction 1 | Wild-Type Polymeric Fraction 2 | cesa5-1 Total Water-Extracted Mucilage | mum5-1 Total Water-Extracted Mucilage |
| Composition (mg g−1 intact seeds) | |||||
| GalA | 10.6 (1.07) | – | – | 13.1 (1.53) | 14.7 (1.27) |
| Neutral sugars | 9.4 (0.58) | – | – | 11.3 (0.44) | 12.4 (0.62) |
| GalA-neutral sugars | 1.13 (0.055) | – | – | 1.16 (0.092) | 1.18 (0.067) |
| Total sugars | 20.0 (1.63) | – | – | 24.3 (1.97) | 27.1 (1.79) |
| Total polymeric materiala | 22.0 (2.75) | 1.6 (0.10) | 20.4 (2.71) | 27.2 (3.58) | 30.6 (3.08) |
| Macromolecular characteristics | |||||
| Mn (kD) | 815 (95.1) | 29,953 (2623) | 754 (81.0) | 448 (45.9) | 422 (50.9) |
| Mp (kD) | 659 (90.9) | 32,650 (3519) | 659 (90.9) | 453 (12.7) | 450 (7.5) |
| Mw (kD) | 3,180 (587.8) | 30,593 (2763) | 911 (98.1) | 667 (167.7) | 650 (202.1) |
| I (Mw/Mn) | 3.9 (0.32) | 1.0 (0.00) | 1.2 (0.04) | 1.5 (0.68) | 1.5 (0.31) |
| [η] (mL g−1) | 601 (8.3) | 808 (10.7) | 583 (10.4) | 594 (12.3) | 633 (16.9) |
| a | – | – | 0.70 (0.166) | 0.69 (0.120) | 0.75 (0.145) |
a
From HP-SEC refractometric signal, using dn/dc = 0.146.