Phenotypic analysis and mendelization of the locule number QTL (lc). A, Fruit morphology of lines used in the study. The parental lines Cervil and Levovil were crossed to obtain the F1 hybrid and the near-isogenic recombinant F8 lines CF12-C and CF13-L. The L2 line was derived from Levovil, and its lc region of chromosome 2 containing the Cervil allele has been introgressed by marker-assisted selection. B, Phenotypic effect of the QTL. The average locule number of F2 heterozygous plants (H) or F3 plants homozygous for either the Cervil (C) or the Levovil (L) allele was determined. The plants were selected based on genotyping data, and heterozygous plants were selected by analyzing genotypic and phenotypic segregation in their corresponding F3 progeny. Each histogram, with its sd, is partitioned according to the proportion of fruits with two locules (white), three locules (black), and four or more locules (gray). A Student’s t test indicated that the means are significantly different between C and H (P < 0.001), between H and L (P < 0.001), and between H and C (P < 0.01). [See online article for color version of this figure.]