Figure 2.

Sumoylation in roots under conditions of excess Cu. Western-blot analysis was performed with homemade anti-SUMO1 antibody. A, Sumoylation in roots of 12-d-old wild-type (WT) and siz1-2 seedlings treated for 1 h with half-strength MS solution (control [C]) or half-strength MS solution containing 10, 25, or 50 μm CuSO₄ or subjected to heat shock at 37°C for 1 h. Loadings of heat shock samples are one-fifth of control and excess Cu-treated samples. B, Sumoylation of root proteins isolated from plants treated with 50 μm CuSO₄ for the indicated time periods. Asterisks indicate unidentified protein. Bottom panels show a gel portion stained with Coomassie blue.