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Cu content in shoot and root tissues. One-week-old wild-type (WT) and siz1-2 seedlings were treated with excess Cu (25 or 35 μm CuSO4, Cu25, and Cu35) for 10 d. Cu concentrations in the shoot (top panel) and root (middle panel) were determined by ICP-OES. In the bottom panel, calculated shoot-to-root ratios of Cu concentration are shown. Means and error bars were calculated from three repeats (* P < 0.05, ** P < 0.01). DW, Dry weight.
