Figure 5.

Differential expression of Cu-related transporter genes in siz1. Twelve-day-old wild-type (WT) and siz1-2 plants were treated with half-strength MS medium or half-strength MS medium with 25 μm CuSO₄ (Cu₂₅) for 1 d. Gene expression of Cu homeostasis-related transporters in the shoot and root tissues was measured by qPCR. The y axis values represent expression relative to ACT2. Means and error bars were calculated from six samples of two biological repeats. Asterisks indicate genes with an expression ratio greater than 2 or lower than 0.5 for siz1:the wild type).