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. 2011 Jun 13;156(4):1921–1933. doi: 10.1104/pp.111.175810

Figure 1.

Figure 1.

Construction of plasmid vectors, the mRNA expression of AtP5CS1, and determination of free Pro content. A, Schematic presentation of vectors pHspGUS and pHspP5CS. LB, Left border; RB, right border. B, Expression level of AtP5CS1 in wild-type (WT), HspGUS4, and transgenic lines, as determined by semiquantitative RT-PCR. C, Measurement of free Pro content in all stages. FW, Fresh weight. D, Schematic drawing of the experimental design for HS. CK, Control; IND, Pro induction at 37°C for 24 h; HS, heat stress at 50°C for 4 h after Pro induction; RE, recovery from HS. For semiquantitative RT-PCR analysis and Pro accumulation measurements, seedlings were recovered for 24 h. The IND and HS treatments started after 8 h of light, with the same photoperiods and light conditions as for the control. Results were calculated from measurements of at least three repeats.