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. 2011 May 27;156(4):2026–2036. doi: 10.1104/pp.111.180042

Figure 3.

Figure 3.

CMV-HL2b interacts with Arabidopsis CAT3. A, Schematic representation of the C-terminal deletion mutants of HL2b for the yeast two-hybrid assay. Deleted forms of the HL2b gene were fused to the binding domain in the pGBKT7 vector (Clontech), and the CAT3 ORF was inserted in pGADT7 activation domain vector (Clontech). Transcriptional activity of β-galactosidase was assayed. Plus (+) indicates transcriptional activation, while minus (−) indicates a negative result. aa, Amino acids. B, Symptoms in the Col-0 plants infected with C2-H1-HL2b or C2-H1-HL2bΔ40. The recombinant HL2bs (HL2b, HL2bΔ12, HL2bΔ33, and HL2bΔ40) were inserted in the CMV vector, C2-H1, for virus inoculation experiments. No necrotic symptom was observed in the Col-0 plants infected with C2-H1-HL2bΔ40. C, CMV accumulation levels. ELISA was conducted to analyze the CMV accumulation in Col-0 plants. Error bars represent se of the means. OD405, Optical density at 405 nm. [See online article for color version of this figure.]