Table III. Summary of TGICL merged super assembly validation using 454 sequence data.

Parameter	MIRA+Newbler	TGICL+Newbler	MIRA+TGICL	MIRA+TGICL+Newbler
Contigs (100 bp or greater)a	20,918	20,639	32,654	34,314
Singletons (100 bp or greater)b	27,781	18,981	17,854	17,020
Total contigs/singletons (100 bp or greater)	48,699	39,620	50,508	51,334
Assembly size (Mb)	54.3	45.3	54.2	58.2
Large contigs (1,000 bp or greater)	19,367	17,007	19,226	20,986
Maximum contig length (bp)	16,338	15,659	16,598	16,333
Average contig length (bp)	1,115	1,144	1,072	1,133.2
N50 (bp)	1,494	1,552	1,406	1,517
Contigs with significant hits (%)c	40,874 (83.9)	26,933 (68.0)	41,556 (82.3)	42,488 (82.8)
Contigs showing 80% or greater coverage (%)d	12,896 (26.5)	11,993 (30.3)	12,270 (24.3)	13,572 (26.4)
Soybean protein hits (%)e	20,954 (45.2)	17,560 (37.9)	21,188 (45.7)	20,987 (45.2)
Soybean proteins with 80% or greater coverage (%)f	10,485 (22.6)	10,314 (22.2)	10,275 (22.2)	10,780 (23.2)
No. of 454 reads mapped (%)	1,740,301 (90.1)	1,815,449 (94.0)	1,745,431 (90.4)	1,840,977 (95.3)
No. of 454 reads uniquely mapped (%)	1,214,991 (62.9)	1,401,916 (72.6)	1,262,790 (65.4)	1,179,585 (61.1)

^aContigs generated by TGICL in merged super assembly.

^bContigs of primary assembly not assembled by TGICL in merged super assembly.

^cContigs showing significant hits (E ≤ 1e-5) with soybean proteins.

^dContigs showing 80% or greater coverage of soybean proteins.

^eUnique soybean proteins to which contigs show significant hits (E ≤ 1e-5).

^fUnique soybean proteins to which contigs show 80% or greater coverage.