Table IV. Summary of TGICL hybrid assembly validation.
| Parameter | MIRA+Short-Read | TGICL+Short-Read | Newbler+Short-Read |
| Contigs (100 bp or greater)a | 30,432 | 21,064 | 18,968 |
| Singletons (100 bp or greater)b | 21,361 | 22,027 | 15,792 |
| Total contigs/singletons (100 bp or greater) | 51,793 | 43,091 | 34,760 |
| Assembly size (Mb) | 51.99 | 43.61 | 35.47 |
| Large contigs (1,000 bp or greater) | 18,047 | 16,038 | 13,803 |
| Maximum contig length (bp) | 16,616 | 15,602 | 15,661 |
| Average contig length (bp) | 1,004 | 1,012 | 1,020 |
| N50 (bp) | 1,424 | 1,485 | 1,671 |
| Contigs with significant hits (%)c | 41,857 (80.8) | 29,194 (67.7) | 27,317 (78.6) |
| Contigs showing 80% or greater coverage (%)d | 12,644 (24.4) | 12,375 (28.7) | 11,952 (34.4) |
| Soybean protein hits (%)e | 21,584 (46.5) | 18,904 (40.8) | 18,514 (39.9) |
| Soybean proteins with 80% or greater coverage (%)f | 10,855 (23.4) | 11,097 (23.9) | 10,683 (23.0) |
| No. of 454 reads mapped (%) | 1,758,638 (91.1) | 1,834,911 (95.0) | 1,720,477 (89.1) |
| No. of 454 reads uniquely mapped (%) | 1,350,782 (69.9) | 1,538,879 (79.7) | 1,576,415 (81.6) |
| No. of short reads mapped (%) | 94,674,527 (88.8) | 95.972,813 (90.0) | 94,217,680 (88.3) |
| No. of short reads uniquely mapped (%) | 47,020,498 (44.1) | 63,410,043 (59.5) | 79,718,851 (74.7) |
a
Contigs generated by TGICL in hybrid assembly.
b
Contigs of primary assembly not assembled by TGICL in hybrid assembly.
c
Contigs showing significant hits (E ≤ 1e-5) with soybean proteins.
d
Contigs showing 80% or greater coverage of soybean proteins.
e
Unique soybean proteins to which contigs show significant hits (E ≤ 1e-5).
f
Unique soybean proteins to which contigs show 80% or greater coverage.