| Protein sample /Technical parameters | GluFibrino-peptidemonomer1.6 kDa | Myoglobinmonomer17 kDa | Hemoglobintetramer67 kDa | Transferrinmonomer80 kDa | GroEL14-mer801 kDa |
| Backing pressure, mBar | 4.4 | 5.0 | 5.1 | 5.1 | 6.5 |
| Trap pressure, mBar | 1.6x10-2 | 2.4x10-2 | 2.4x10-2 | 2.6x10-2 | 2.8x10-2 |
| IMS Pressure, mBar | 4.4x10-1 | 4.4x10-1 | 4.4x10-1 | 4.4x10-1 | 4.2x10-1 |
| Sampling cone voltage, V | 46 | 80 | 80 | 80 | 118 |
| Extraction cone voltage, V | 1.7 | 1 | 1 | 1 | 3 |
| Bias voltage, V | 20 | 20 | 25 | 25 | 50 |
| Trap collision energy, V | 20 | 15 | 15 | 15 | 80 |
| Transfer collision energy, V | 5 | 12 | 12 | 12 | 15 |
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