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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Atherosclerosis. 2011 Jun 23;217(2):407–414. doi: 10.1016/j.atherosclerosis.2011.06.031

Figure 2. Effects of statins on cellular cholesterol level, expression of the sterol-related genes and ABCA7 protein level.

Figure 2

A: J774 cells were subcultured in a 6-well tray as 1 × 106 cells/well for one day, and incubated overnight with and without 50 μM pravastatin (P50), 5 μM rosuvastatin (R5), or 5 mM cyclodextrin (CD5). Cell lipid was extracted and cholesterol was measured. B - G: J774 cells subcultured as above were incubated overnight with and without 50 μM pravastatin (B, E), 5 μM rosuvastatin (C, F), and 5 mM cyclodextrin (D, G). mRNA was extracted and quantitative expression analysis was performed for ABCA1, ABCA7, LXRα, SREBP1, SREBP2, the LDL receptor (LDLR) and standardized for β-actin (B, C, D). Results were expressed as fold change relative to the each control. The protein was extracted and analyzed for ABCA7 by Western blotting (E, F, G). Data represent the mean ± SD for six (A) and eighteen (B - G) samples. Statistical significance is indicated as * for P < 0.05, ** for P < 0.01 and *** for P < 0.001 against each control.