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. Author manuscript; available in PMC: 2011 Aug 4.
Published in final edited form as: Cell. 2010 Apr 30;141(3):483–496. doi: 10.1016/j.cell.2010.03.040

Figure 1. Domain organization, expression and intracellular localization of human and mouse NLRC5.

Figure 1

(A) Domain organization of human and mouse NLRC5 proteins. CARD, caspase recruitment domain; NOD, nucleotide binding domain; LRR, leucine rich repeats.

(B) Western blot analysis of HA-tagged NLRC5 and mNLRC5 protein expression in 293T cells.

(C) Human and mouse NLRC5 mRNAs were determined in different tissues determined by real-time PCR analysis.

D) Expression of mNLRC5 mRNA in RAW264.7 cells was determined by real-time PCR analysis after LPS, intracellular poly (I:C) stimulation or VSV-eGFP infection.

(E) Immunoblot analysis of mNLRC5 protein expression after LPS stimulation or VSV-eGFP infection. The relative protein levels of mNLRC5 were quantified by the band density scanning of Western blot images and plotted against time (h) after LPS treatment or VSV infection.

(F) Both wild-type and MyD88-deficient macrophages were treated with LPS, and mNLRC mRNA expression was determined by real-time PCR analysis.

See also Fig. S1.