(A) Four NLRC5 deletion constructs were generated.
(B) and (C) 293T cells were transfected with NLRC5 and its mutations constructs, IKKβ or kinase domain of IKKβ and analyzed by coimmunoprecipitation and Western blot.
(D) 293T cells were transfected with NF-κB-luc reporter, together with an empty vector, or with full-length NLRC5 and its deletion constructs, and analyzed for luciferase activity (fold induction).
(E) 293T cells were transfected with various expression plasmids and the phosphorylation of IKKα or IKKβ was determined.
See also Fig. S4G.