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. Author manuscript; available in PMC: 2012 Aug 25.
Published in final edited form as: Neuroscience. 2011 May 27;189:123–131. doi: 10.1016/j.neuroscience.2011.05.020

Figure 2. Effect of TGF-β1 on expression of neuronal-specific markers by RGC-5 cells.

Figure 2

Western blot analysis for: (A) Brn-3c, (B) NF-160 and (C) Thy1.2 and realtime-PCR for: (D) Thy1.2, (E) Tau and (F) PGP9.5. Brn-3c and NF-160 were not detected in undifferentiated RGC-5 but were expressed by differentiated RGC-5. Thy1.2 was present in undifferentiated RGC-5s and increased after differentiation. Differentiation increased expression of Thy1.2, Tau and PGP9.5. Addition of TGF-β1 to differentiated RGC-5 cells increased NF-160, but not Brn-3c or Thy1.2 protein and increased PGP9.5, but not (D) Thy1.2 or (E) Tau mRNA. N = 3; *p<0.05