Figure 2.

Quantitative analysis of Ixosc SULT 1 and SULT 2 mRNA Expression. Total RNA was extracted from different developmental stages and PCR amplified with gene specific primers. Quantitative transcriptional expression was determined as described in Materials and Methods. The PCR rates of SULT 1 (A) and SULT 2 (B), in various stages of the tick life cycle were normalized to the rate of synthesis of β-actin, included as the endogenous control. Data were plotted as copy numbers per reaction to samples at various level of feeding. Bars indicate PCR values as the Mean ± SD of three replicated experiments. Abbreviations: UL - Unfed Larvae, FL - Fed Larvae, UN - Unfed Nymph, FN - Fed Nymph.