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. 2011 Jun 27;12:32. doi: 10.1186/1471-2091-12-32

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Copyright ©2011 Pichu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of Ixosc Sult 2 protein expression during feeding. (A) Ixosc Sult 2 detected by Western blot, (B) Quantitation of band intensity. Tissue homogenates from different developmental stages were analyzed by Western blot using anti-Sult 2 (R) raised antibody. Lanes: 1. Magic Marker, 2. Unfed larvae, 3. Fed larvae, 4. Unfed nymph, 5. Fed nymph. SULT 2 gene products were quantified using Kodak Digital Science 1D image analysis software and the net intensity/band intensity was plotted against the different tick stages.