Table 1.

Quantitation of Ixosc Sult 1 (R) and 2 (R) activity.^a

Reaction mixture	Rate of reaction (nmol min-1 mg-1)
PAP35S + Sult 1 (R) (Control)	0.00
p-Nitrophenol + Sult 1 (R) + PAP35S	1.30 ± 0.01
Dopamine + Sult 1(R) + PAP35S	1.52 ± 0.01
Octopamine + Sult 1(R) + PAP35S	1.34 ± 0.01
PAP35S + Sult 2 (R) (Control)	0.00
p-Nitrophenol + Sult 2 (R) + PAP35S	0.43 ± 0.01
Dopamine + Sult 2 (R) + PAP35S	0.39 ± 0.01
Octopamine + Sult 2 (R) + PAP35S	0.35 ± 0.01

^a Substrates (10 μM) were incubated with PAP³⁵S (4 μM) and purified Ixosc Sult 1 (R)(0.04 mg/mL) or Ixosc Sult 2 (R)(0.03 mg/mL) for 30 min. Product formation in the absence of enzyme (PAP³⁵S + substrate) was found to be zero.