Figure 2. S-Ag specific T cell responses of immunized HLA class II Tg mice.

EAU susceptible and resistant HLA class II transgenic strains were immunized with a uveitogenic regimen of S-Ag. Proliferative and cytokine responses of cells from lymph nodes draining the site of immunization were examined. (A) Draining lymph node cells collected 30 days after immunization were cultured with serial dilutions of S-Ag. Average background H³ uptake by the cells in the absence of antigen was approximately 4000 cpm and is subtracted (representative data from one of two experiments). (B) Cytokine production was measured by multiplex ELISA, in cell culture supernatants collected 48 hrs after in-vitro antigenic stimulation. Antigen specific cytokine production is plotted after subtraction of the background levels in the absence of antigen (representative data from one of three experiments).