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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1977–1985. doi: 10.4049/jimmunol.1101247

Figure 3. Immunodominant epitope profiles of human S-Ag protein for the susceptible HLA class II alleles.

Figure 3

Spleen cells from S-Ag immunized mice were used in an in-vitro lymphocyte proliferation assay in the presence of 25µM of each of 20 amino acid long overlapping synthetic 20mer peptides spanning the human S-Ag protein sequence, peptide M, peptide N, and whole S-Ag protein (20µg/ml). [3H] Thymidine uptake by cells was plotted after subtracting the background counts in cells cultured in the absence of antigen (average background counts ≤ 5000 cpm). Data shown is the average of five individual mice for each strain.