Figure 1. GmaR protein is degraded at 37°C and in the absence of MogR.

GmaR protein levels were determined following growth in the presence of tetracycline and upon temperature shift. Wild-type (WT) and ΔmogR bacteria were grown 16–18 h at room-temperature (RT: 22°C–24°C) without shaking. Cultures were diluted to an OD₆₀₀ = 0.4, split into two cultures and 8 µg/ml tetracycline was added. Cultures were then shifted to either 30°C or 37°C and grown without shaking for an additional 8 h. Samples were taken every hour for determination of OD₆₀₀, cfu/mL (Figure S1) and Western blot analysis. (A) Western blot analysis of GmaR and DegU (control). (B) Quantitative analysis of Western blots. Densitometry of Western blots from three independent experiments as described above was performed. For each condition, the percentage of GmaR protein present at each time point following the addition of tetracycline in relation to GmaR present at T = 0 is shown. The intersection of the dashed line represents the time at which 50% of the initial GmaR protein was present. Data represent the means and standard deviations of three independent experiments.