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. 2011 Aug 4;7(8):e1002153. doi: 10.1371/journal.ppat.1002153

Figure 3. MogR binds to flagellar gene promoter region DNA in a temperature-independent manner.

Figure 3

Gel mobility shift analysis of MogR binding to fliN-gmaR (A) and flaA (B) promoter region DNA. Radiolabeled promoter region DNA fragments were incubated at either 30°C or 37°C with increasing concentrations of MogR-His6. Binding reactions were separated by non-denaturing PAGE and detected by autoradiography. Shifted (S), supershifted (SS), and super-supershifted (SSS) DNA complexes are indicated.