Figure 4. Formation of the MogR:GmaR complex is more efficient at 30°C than at 37°C.

Gel mobility shift analysis of MogR binding to fliN-gmaR (A) and flaA (B) promoter region DNA. Radiolabeled promoter region DNA fragments were incubated at either 30°C or 37°C with 40 nM MogR-His₆ for 30 min. GmaR-His₆ was then added at increasing concentrations and incubated for an additional 30 min at the indicated temperature. Binding reactions were separated by non-denaturing PAGE and detected by autoradiography. Shifted (S), supershifted (SS), and super-supershifted (SSS) DNA complexes are indicated.