Skip to main content
. 2011 Aug 4;7(8):e1002190. doi: 10.1371/journal.ppat.1002190

Figure 4. Rgg3 binds to shp3 and shp2 promoter regions but can be disrupted by cognate SHPs.

Figure 4

EMSA analysis was used to test the ability of recombinant Rgg3 to bind to target promoters and the effect of SHPs on this binding. (A) Rgg3 binds in a concentration-dependent manner to the promoters of both shp3 (upper panel) and shp2 (lower panel). (B) Binding to Pshp3 by Rgg3 is disrupted by the addition of pure (>95%) sSHP3-C8, and to a lesser extent by pure sSHP2-C8. Both peptides had a smaller effect on disruption of binding to Pshp2. Pure sSHP3-C8-rev was included as a control and did not affect binding to either probe. (C) Binding of Pshp3 and Pshp2 by Rgg3 is specific and can be disrupted by addition of 5-fold molar excess of unlabeled specific probe but not an equivalent amount of unlabeled rRNA probe (nonspecific). No binding of a negative control rRNA probe (PrRNA) was detected. All reactions contained 10 nM probe, and 50 nM unlabeled competitor where indicated. Protein concentration is indicated above each lane in all panels.