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. 2011 Aug 4;7(8):e1002190. doi: 10.1371/journal.ppat.1002190

Figure 5. Proteins previously identified in peptide signaling circuits are also important for Rgg-dependent gene regulation.

Figure 5

The oligopeptide permease and Eep protease are important for SHP signaling. (A) A ΔoppD mutant (JCC163) fails to respond to 50 nM sSHP3-C8 unless an oppD complementation vector, pOppD, is present. A wild-type reporter strain carrying empty vector (JCC157 (pLZ12-Sp)) was included as a control. (B) Expression of the C-terminus of SHP3 from a plasmid (pSHP315-23) bypasses the need for the permease in the ΔoppD mutant (JCC163). Wild-type (JCC157) and the Δrgg3 (JCC158) reporter strains carrying the vector (pLZ12-Sp) alone are included for reference. (C) The Eep metalloprotease contributes to signal production, as determined by measuring luciferase activity of the Δrgg3Δeep mutant (JCC161) expressing eep from a multi-copy plasmid (pEep) or carrying empty vector (pLZ12-Sp); Δrgg3 (JCC158) was included as a control. (D) Overexpression of shp3 (pSHP3) restores light induction in the Δeep mutant (JCC160) to near wild-type (JCC157) levels. Data shown are representative of experiments performed at least three times.